Extended-spectrum beta-lactamase and Antibiotic susceptibility pattern in uropathogens.

Objective: To identify rapidly changing antibiotic susceptibility of ESBL & Uropathogens. Study Design: Comparative Cross-sectional prospective study. Setting: Allied Hospitals Peshawar Medical College. Period: 8th November 2021 to 5th March 2022. Material Methods: 10-15ml midstream urine was collected in a sterile container from 158 patients Pus cells, red blood and bacteria were examined using microscopy. The standard loop technique used inoculate specimen on MacConkey Blood agar. Plates incubated for 24 hours at 37oC. A colony count 105 cfu/ml thought be significant. Gram staining the colonies performed. biochemical tests conducted API 10S identification organisms. Extended-spectrum beta-lactamase organisms identified by double-disc synergy method. Kirby-Bauer disc diffusion method test Mueller-Hinton agar according CLSI guidelines 2021. statistical package social sciences (SPSS) version 20.0 analysis. Results: Out samples 135 (85.5%) had positive culture growth with 35 (25.9%) confirmed. Antibiotic Nitrofurantoin (70.1%), Trimethoprim-sulfamethoxazole (26.8%), Ciprofloxacin (51.5%), Levofloxacin Ceftriaxone (25.77%) Cefotaxime (23.7%) Ceftazidime (19.5%) Cefepime (27.8%) Aztreonam (2.1%) Meropenem (86.6%) Amoxicillin/clavulanic (37.1%) Gentamycin (73%) Penicillin (0%). Conclusion: Surprisingly, only nitrofurantoin found advised orally as suitable drug treatment UTIs among 13 commonly antibiotics.